N-Acetylglucosamine-1-phosphate

HPLC Method for Analysis of N-Acetylglucosamine-1-phosphate, N-Acetylglucosamine-6-phosphate on Newcrom B by SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of N-Acetylglucosamine-1-phosphate, N-Acetylglucosamine-6-phosphate

N-Acetylglucosamine-6-phosphate (GlcNAc-6-P) and N-Acetylglucosamine-1-phosphate (GlcNAc-1-P) are two phosphorylated derivatives of N-acetylglucosamine (GlcNAc), a monosaccharide that is part of various biological structures, such as glycoproteins and glycosaminoglycans.

1. N-Acetylglucosamine-6-phosphate (GlcNAc-6-P):
   • This molecule is an intermediate in the biosynthesis of glycosaminoglycans like hyaluronic acid and chondroitin sulfate.
   • It is involved in several metabolic pathways, including those related to the formation of UDP-GlcNAc, a precursor for glycosylation reactions.
   • The 6-phosphate group is added to the carbon-6 position of the GlcNAc molecule.
2. N-Acetylglucosamine-1-phosphate (GlcNAc-1-P):
   • This compound is an important intermediate in the synthesis of glycoproteins and glycosaminoglycans.
   • It plays a role in the biosynthesis of UDP-GlcNAc, which is used in glycosylation processes.
   • The 1-phosphate group is attached to the carbon-1 position of the GlcNAc molecule.
   • This compound can also participate in the formation of glycosaminoglycans like heparan sulfate and chondroitin sulfate.

Both GlcNAc-6-P and GlcNAc-1-P are involved in the regulation of metabolism and glycosylation, but they differ in the position of the phosphate group on the glucose structure.

N-Acetylglucosamine-6-phosphate (GlcNAc-6-P) and N-Acetylglucosamine-1-phosphate (GlcNAc-1-P) can be retained, separated and analyzed using an Newcrom B mixed-mode stationary phase column. The analysis employs a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and formic acid as a buffer. Detection is achieved using LC MS.