HPLC Method For Analysis Of Histidine and Histidine Methyl Ester Dihydrochloride on Primesep 100 Column

Separation type: Liquid Chromatography Mixed-mode

 


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High Performance Liquid Chromatography (HPLC) Method for Analysis of Histidine and Histidine Methyl Ester Dihydrochloride

Histidine and Histidine Methyl Ester Dyhydrochloride are related compounds with interesting uses. Histidine is used in biosynthesis to create proteins, while histidine methyl ester dihydrochloride (a derivative of histidine) is used to create various other compounds.
This amino acid and its derivative can be detected in the low UV regime. Using a Primesep 100 mixed-mode column and a mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid (H2SO4) buffer, Histidine and Histidine Methyl Ester Dyhydrochloride can be retained, separated, and analyzed. This analysis method can be UV detected at 200 nm.

Condition

Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 60/40%
Buffer H2SO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV, 200 nm

 

Description

Class of Compounds
Amino acid
Analyzing Compounds Histidine,  Histidine Methyl Ester

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Histidine
Histidine methyl ester
L-Histidine hydrochloride monohydrate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.