| CAS Number | 220127-57-1 |
|---|---|
| Molecular Formula | C30H35N7O4S |
| Molecular Weight | 589.720 |
| InChI Key | YLMAHDNUQAMNNX-UHFFFAOYSA-N |
| LogP | 2.84 |
| Synonyms |
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Applications:
Determination Imatinib Mesylate on Obelisc R Column
February 10, 2021
Separation type: Liquid Chromatography Ion Exclusion
High Performance Liquid Chromatography (HPLC) Method of Imatinib mesylate.
Imatinib mesylate is effective in treating chronic myeloid leukemia and metastatic gastrointestinal stromal tumors. It is classified as a signal transduction inhibitor. It can be retained on an Obelisc R column, which has both positive and negative ion-pairs embedded in the stationary phase, allowing for the fine tuning and separation of a wide range of compounds with different ionic properties. Imatinib mesylate can be determined isocratically using a simple MS-compatible mobile phase of acetonitrile (ACN) and water with Ammonium Formate (AmFm) buffer and detected by UV at 270nm, ELSD, CAD or LC/MS.
| Column | Obelisc R, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN -50% |
| Buffer | Ammonium Formate pH 3.0 – 50 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 270nm |
| Class of Compounds |
Drug |
| Analyzing Compounds | Imatinib Mesylate |
Application Column
Obelisc R
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
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Separation of Imatinib mesylate on Newcrom R1 HPLC column
May 16, 2018
Imatinib mesylate can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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