Determination Imatinib Mesylate on Obelisc R Column

Separation type: Liquid Chromatography Ion Exclusion

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High Performance Liquid Chromatography (HPLC) Method of Imatinib mesylate. Imatinib mesylate is effective in treating chronic myeloid leukemia and metastatic gastrointestinal stromal tumors. It is classified as a signal transduction inhibitor. It can be retained on an Obelisc R column, which has both positive and negative ion-pairs embedded in the stationary phase, allowing for the fine tuning and separation of a wide range of compounds with different ionic properties. Imatinib mesylate can be determined isocratically using a simple MS-compatible mobile phase of acetonitrile (ACN) and water with Ammonium Formate (AmFm) buffer and detected by UV at 270nm, ELSD, CAD or LC/MS.

Condition

Column Obelisc R, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN -50%
Buffer Ammonium Formate pH 3.0 - 50 mM
Flow Rate 1.0 ml/min
Detection UV 270nm
 

Description

Class of Compounds  Drug
Analyzing Compounds Imatinib Mesylate
 
Application Analytes:
Imatinib mesylate