HPLC Method for Analysis of Fluroxypyr-meptyl on Primesep 100 Column

HPLC Method for Separation of Fluroxypyr-meptyl on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of Fluroxypyr-meptyl on Primesep 100 Column by SIELC Technologies

Fluroxypyr-meptyl is a herbicide used for the control of broad-leaved weeds in cereals, pastures, and non-crop areas. It belongs to a class of herbicides known as synthetic auxins.

Auxins are a class of plant hormones which are involved in the regulation of plant growth. Synthetic auxins like fluroxypyr mimic the action of natural auxins but disrupt the normal growth processes in the target plants, leading to their death.

Fluroxypyr-meptyl is effective against a range of weed species, including some that are resistant to other types of herbicides.

This herbicide can be retained and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Sulfuric acid (H2SO4). This analytical method can be UV detected at 220 nm with high resolution and peak symmetry.

LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Fluroxypyr-meptyl


ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 70/30%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 220 nm
Peak Retention Time3.82 min
LOD0.1 ppm


Class of CompoundsHerbicide
Analyzing CompoundsFluroxypyr-meptyl

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.