HPLC Separation of Denatonium benzoate on Newcrom A and Primesep SB Column


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Condition 1

Column Newcrom А, 3.2×50 mm, 3 µm, 100A
Mobile Phase MeOH/H2O – 20/78.4%
Buffer H2SO4 – 1.6%
Flow Rate 0.5 ml/min
Detection UV 275 nm

 

Denatonium is the most bitter chemical compound known.
Denatonium benzoate is a substance that’s put into household, garden and automotive products to ensure that if a child or animal ever tried to swallow it, they would spit it straight out before they could be poisoned.

 

Condition 2

Column Primesep SB, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 10-90%, 10 min
Buffer Gradient H2SO4 – 0.9-0.1%, 10 min
Flow Rate 1.0 ml/min
Detection UV, 275 nm

 

Description

Class of Compounds
Drug, The bitter chemical compound, Basic, Hydrophobic, Ionizable
Analyzing Compounds Denatonium benzoate

 

Application Column

Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Newcrom A

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Benzoic Acid
Denatonium benzoate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.