HPLC Method for Separation of Benzonitrile, Toluene and Benzylamine on Primesep A Column

HPLC Method for Analysis of Benzonitrile, Toluene, Benzylamine on Primesep A by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Separation of Benzonitrile, Toluene and Benzylamine on Primesep A Column by SIELC Technologies
  1. Benzonitrile: Benzonitrile (C₆H₅CN) is an aromatic organic compound. It is a colorless liquid with an almond-like odor. Benzonitrile is used as a solvent and a chemical intermediate in the production of a variety of compounds, including pharmaceuticals, dyes, and perfumes.
  2. Toluene: Toluene (C₇H₈) is a colorless, water-insoluble liquid with the smell associated with paint thinners. It is a mono-substituted benzene derivative, consisting of a CH₃ group attached to a phenyl group. It is an important organic solvent and is also used to produce benzene, and a number of consumer products including certain types of glue, paint thinners, and nail polish removers.
  3. Benzylamine: Benzylamine (C₆H₅CH₂NH₂) is a primary amine compound containing a benzyl group attached to the amino group. It is a colorless liquid with a faint almond-like odor. It is used as a chemical intermediate in the synthesis of a variety of substances, including pharmaceuticals, dyes, and polymers.

These chemicals are widely used in organic synthesis. Please handle these substances with care, as they can have hazardous effects. Always refer to the safety data sheets for each chemical and adhere to recommended safety procedures.

These three compounds be separate and analyzed on a reverse-phase Primesep A, 4.6 x 150 mm, 5 µm, 100 A column with a mobile phase consisting of water, Acetonitrile (MeCN) and Sulfuric acid as a buffer modifier. This analysis method can be UV detected at 210 nm.

LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Benzonitrile, Toluene, Benzylamine


ColumnPrimesep A, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O -60/40%
Flow Rate1.0 ml/min
DetectionUV 210 nm
Peaks Retention Time2.9, 4.0, 10.2 min
Samples concentration (Benzonitrile, Toluene, Benzylamine)0.04, 0.05, 0.07 mg/ml
Injection volume3 µl
Sample diluent MeCN/H2O -60/40%
LOD (Benzonitrile, Toluene, Benzylamine)27, 65, 103 ppb


Class of CompoundsBenzene, Aromatics
Analyzing CompoundsBenzonitrile, Toluene, Benzylamine

Application Column

Primesep A

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.