HPLC Method for Separation of Levamisole and Mebendazole in Pharmaceutical Formulations on Primesep 100  Column

HPLC Method for Separation of Levamisole, Mebendazole on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Separation of Levamisole and Mebendazole in Pharmaceutical formulations

Levamisole and Mebendazole are both anthelmintic drugs, meaning they are used to treat parasitic worm infections. Each works in a different way and is used to treat different types of infections:

  1. Levamisole: This medication is used primarily to treat worm infections. It works by paralyzing the worms which are then expelled from the body by the immune system. In the past, it was also used as an immune stimulant in some types of cancer and rheumatoid arthritis. However, its use for these conditions has decreased due to the risk of serious side effects.
  2. Mebendazole: Mebendazole is also an anthelmintic medication used to treat a variety of parasitic worm infections, including roundworm, hookworm, whipworm, and pinworm. Mebendazole works by preventing the worms from absorbing sugars which they need to survive. This kills the worm within a few days. Although this medicine can be used for children, it should not be given to children under 2 years old.

Both drugs are typically taken orally, often as a single dose, but the exact dosage and length of treatment depend on the type of infection being treated. Like all medications, Levamisole and Mebendazole have potential side effects, and they should be used under the supervision of a healthcare provider. It’s also important to remember that hygiene practices are crucial in preventing reinfection with worms, especially in areas where such infections are common.

These compounds can be retained, separated, and analyzed using a reverse-phase Primesep 100, 3.2 x 100 mm, 5 µm, 100 A column. The mobile phase for this method consists of water, acetonitrile (MeCN), and Sulfuric acid, which serves as a buffer. This analytical method can be

High Performance Liquid Chromatography (HPLC) Method for Analysis of Levamisole, Mebendazole

Condition

ColumnPrimesep 100, 3.2 x 100 mm, 5 µm, 100 A
Mobile PhaseMeCN -30%
BufferH2SO4 – 0.5%
Flow Rate0.5 ml/min
DetectionUV 210 nm

Description

Class of CompoundsDrug
Analyzing CompoundsLevamisole, Mebendazole

Application Column

Primesep 100

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Levamisole
Mebendazole
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.