HPLC Method for Separation of Tribenzylamine on Primesep B by SIELC Technologies

Tribenzylamine, also written as TBA, is an organic compound with the chemical formula C21H21N. It falls under the class of triarylamines. Triarylamines are compounds containing an amine group (-NH2) attached to three aromatic groups (in this case, benzyl groups). In the case of tribenzylamine, the amine nitrogen is attached to three benzyl groups, which are phenyl (benzene) rings attached to a methylene (-CH2-) group. It’s often used in organic chemistry as a reagent, particularly in the production of other complex organic compounds.
The Tribenzylamine can be retained and analyzed using a mixed-mode Primesep B column. The mobile phase for this method consists of water, acetonitrile (MeCN), and Sulfuric acid, which serves as a buffer. This analytical method can be monitored using UV detection at 200 nm.
Condition
| Column | Primesep B, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN -10% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 200 nm |
Description
| Class of Compounds | Triarylamines |
| Analyzing Compounds | Tribenzylamine |
Application Column
Primesep B
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended



