HPLC Method for Analysis of Tribenzylamine (TBA)  on Primesep B Column

HPLC Method for Separation of Tribenzylamine on Primesep B by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of Tribenzylamine on Primesep B Column by SIELC Technologies
HPLC Method for Analysis of Tribenzylamine (TBA)  on Primesep B Colum

Tribenzylamine (TBA) is an organic compound that falls under the class of triarylamines.

Triarylamines are compounds containing an amine group (-NH2) attached to three aromatic groups (in this case, benzyl groups). In the case of tribenzylamine, the amine nitrogen is attached to three benzyl groups, which are phenyl (benzene) rings attached to a methylene (-CH2-) group.

So, tribenzylamine has the formula (C6H5CH2)3N, with the three benzyl groups making it a tertiary amine.

It’s often used in organic chemistry as a reagent, particularly in the production of other complex organic compounds.

The Tribenzylamine can be retained and analyzed using a mixed-mode Primesep B, 3.2 x 100 mm, 5 µm, 100 A column. The mobile phase for this method consists of water, acetonitrile (MeCN), and Sulfuric acid, which serves as a buffer. This analytical method can be monitored using UV detection at 200 nm.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Tribenzylamine


ColumnPrimesep B, 3.2 x 100 mm, 5 µm, 100 A
Mobile PhaseMeCN -10%
BufferH2SO4 – 0.1%
Flow Rate0.5 ml/min
DetectionUV 200 nm


Class of CompoundsTriarylamines
Analyzing CompoundsTribenzylamine

Application Column

Primesep B

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.