HPLC Method for Analysis of Chlorpheniramine Maleate on BIST B+

HPLC Method for Analysis of Chlorpheniramine Maleate on BIST B+ by SIELC Technologies.

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Chlorpheniramine Maleate on BIST B+ Column
HPLC Method for Analysis of Chlorpheniramine Maleate on BIST B+ by SIELC Technologies.

High Performance Liquid Chromatography (HPLC) Method for Analyses of Chlorpheniramine Maleate

Chlorpheniramine Maleate is a popular antihistamine used to provide relief from the symptoms of allergies, hay fever, and the common cold. Using SIELC’s newly introduced BIST™ method, Chlorpheniramine Maleate, which separates in water, can be retained on a positively-charged anion-exchange BIST™ B column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged analytes to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Chlorpheniramine Maleate can be separated, retained, and UV detected at 210 nm and 275 nm.


Condition

ColumnBIST B+, 4.6×50 mm, 5 µm, 100A
Mobile PhaseMeCN – 85%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 210, 275 nm
Peak Retention Time2.9 min

Description

Class of CompoundsDrug
Analyzing CompoundsChlorpheniramine Maleate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

BIST B

SIELC Technologies’ BIST™ Columns are a new and simple way to achieve many separations that are traditionally difficult or impossible to achieve with any other HPLC columns currently on the market! When used with our BIST™ mobile phases, these ion exchange columns can generate very strong retention of analytes that have the same charge polarity as the stationary phase, unlocking new chromatography applications that were previously too difficult to achieve.

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BIST B+

SIELC Technologies’ BIST™ Columns are a new and simple way to achieve many separations that are traditionally difficult or impossible to achieve with any other HPLC columns currently on the market! When used with our BIST™ mobile phases, these ion exchange columns can generate very strong retention of analytes that have the same charge polarity as the stationary phase, unlocking new chromatography applications that were previously too difficult to achieve.

Select options
Application Analytes:
Chlorpheniramine maleate