Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
Chlorpheniramine Maleate is a popular antihistamine used to provide relief from the symptoms of allergies, hay fever, and the common cold. Using SIELC’s newly introduced BIST™ method, Chlorpheniramine Maleate, which separates in water, can be retained on a positively-charged anion-exchange BIST™ B column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged analytes to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Chlorpheniramine Maleate can be separated, retained, and UV detected at 210 nm and 275 nm.
Column | BIST B+, 4.6×50 mm, 5 µm, 100A |
Mobile Phase | MeCN – 85% |
Buffer | H2SO4 – 0.2% |
Flow Rate | 1.0 ml/min |
Detection | UV 210, 275 nm |
Peak Retention Time | 2.9 min |
Class of Compounds | Drug |
Analyzing Compounds | Chlorpheniramine Maleate |
SIELC Technologies’ BIST™ Columns are a new and simple way to achieve many separations that are traditionally difficult or impossible to achieve with any other HPLC columns currently on the market! When used with our BIST™ mobile phases, these ion exchange columns can generate very strong retention of analytes that have the same charge polarity as the stationary phase, unlocking new chromatography applications that were previously too difficult to achieve.
Select optionsSIELC Technologies’ BIST™ Columns are a new and simple way to achieve many separations that are traditionally difficult or impossible to achieve with any other HPLC columns currently on the market! When used with our BIST™ mobile phases, these ion exchange columns can generate very strong retention of analytes that have the same charge polarity as the stationary phase, unlocking new chromatography applications that were previously too difficult to achieve.
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