HPLC Method for Analysis of Isonicotinic Acid on Primesep 100 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Isonicotinic Acid on Primesep 100 by SIELC Technologies

HPLC Method for Analysis of Isonicotinic Acid on Primesep 100 Column

Isonicotinic Acid is an nucleoside with the C6H5NO2 molecular formula. It is a derivative of pyridine, making it a pyridinemonocarboxylic acid. It acts primarily as a metabolite in humans and algae. You can find detailed UV spectra of Isonicotinic Acid and information about its various lambda maxima by visiting the following link.

Isonicotinic Acid can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 200 nm.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 10%
BufferH2SO4 -0.05%
Flow Rate1.0 ml/min
DetectionUV 200 nm
Samples1.0 mg/ml in MeCN/H2O – 50/50%
Injection volume1 µl
LOD*2 ppb (200 nm)
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Acid
Analyzing CompoundsIsonicotinic Acid

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Isonicotinic Acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.