Separation type: Liquid Chromatography Mixed-mode
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High Performance Liquid Chromatography (HPLC) Method for Analysis of Vecuronium
Vecuronium bromide, also known as Norcuron, is a muscle relaxant used during surgery, intubation, and executions. HPLC methods for Vecuronium are of great interest to pharmaceutical research and industry. Vecuronium bromide can be separated and retained on a mixed-mode stationary phase Primesep SB column with embedded strong basic ion-pairing groups, using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an Ammonium Formate (AmFm) ionic modifier. This analysis method can be UV detected at 260 nm with high resolution and peak symmetry and is compatible with Mass Spectrometry (MS), ELSD, and CAD.
| Column | Primesep SB, 2.1×100 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 10% |
| Buffer | Ammonium Formate pH 3.0 – 50 mM, 100 mM |
| Flow Rate | 0.2 ml/min |
| Detection | ELSD, 50C |
| Class of Compounds | Drug |
| Analyzing Compounds | Vecuronium |
Application Column
Primesep SB
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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