HPLC Method for Simultaneous Determination of Formamide, Propionic Acid and Formic Acid on Newcrom BH Column

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Simultaneous Determination of Formamide, Propionic Acid, Formic Acid on Newcrom BH

High Performance Liquid Chromatography (HPLC) Method for Analysis of Formamide, Propionic Acid, Formic Acid

Formamide is a popular industrial solvent with a wide variety of applications, including in the manufacturing of drugs, herbicides, pesticides, as a paper and fiber softener, and as a solvent for a plethora of ionic compounds. Propionic acid is a popular antifungal and antibacterial compound commonly used in food production as a preservative. Formic Acid is a very popular organic acid with a plethora of industrial applications, including as an agricultural preservative, a key component in fuel cells, and as an ionic modifier in HPLC analysis. These 3 organic acids can be retained, separated, and analyzed on a mixed-mode Newcrom BH column with a mobile phase consisting of water, Acetonitrile (MeCN), and Phosphoric acid (H3PO4). This analytical method can be UV detected at 210 nm with high resolution and peak symmetry.

 

Condition
Column Newcrom BH, 4.6×250 mm, 3 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer H3PO4 – 0.1%
Flow Rate 10 ml/min
Detection UV 210 nm

Description
Class of Compounds Acid, Hydrophilic
Analyzing Compounds Formamide, Propionic Acid, Formic Acid

 

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Formamide
Formic acid
Propionic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.