HPLC Method for Delafloxacin on Primesep 100 by SIELC Technologies
Delafloxacin, also known as Baxdela, is a popular antibiotic with the chemical formula C18H12ClF3N4O4. It is used to treat skin infections from both gram-positive and gram-negative bacteria.
Delafloxacin can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple isocratic mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be UV detected at 290 nm with high resolution and peak symmetry.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 40% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 290 nm |
| Class of Compounds | Drug |
| Analyzing Compounds | Delafloxacin |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
DelafloxacinApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
