HPLC Method For Analysis Of Minocycline on Primesep 100 column

Separation type: Liquid Chromatography Mixed-mode   HPLC.cloud View on hplc.cloud     High Performance Liquid Chromatography (HPLC) Method for Analysis of Minocycline hydrochloride   Minocycline is in a class of medications called tetracycline antibiotics. It works in treatment of infections by preventing the growth and spread of bacteria. Minocycline can be retained on the Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. The analysis method can be UV detected at 270 nm.  


Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 50/50%
Buffer H2SO4 - 0.5%
Flow Rate 1.0 ml/min
Detection UV, 270 nm


Class of Compounds Drug, Antibiotics
Analyzing Compounds Minocycline hydrochloride
Application Analytes:
Minocycline hydrochloride