Separation type: Liquid Chromatography Mixed-mode View on hplc.cloud High Performance Liquid Chromatography (HPLC) Method for Analysis of Minocycline hydrochloride Minocycline is in a class of medications called tetracycline antibiotics. It works in treatment of infections by preventing the growth and spread of bacteria. Minocycline can be retained on the Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. The analysis method can be UV detected at 270 nm.
|Column||Primesep 100, 4.6x150 mm, 5 µm, 100A|
|Mobile Phase||MeCN/H2O - 50/50%|
|Buffer||H2SO4 - 0.5%|
|Flow Rate||1.0 ml/min|
|Detection||UV, 270 nm|
|Class of Compounds||Drug, Antibiotics|
|Analyzing Compounds||Minocycline hydrochloride|