HPLC Method For Analysis Of L-Carnitine and Arginine on Primesep 100 Column

Separation type: Liquid Chromatography Mixed-mode

 


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High Performance Liquid Chromatography (HPLC) Method for Analysis of L-Carnitine and Arginine

L-carnitine is a quaternary ammonium cation (also known as a quat) that is key to the metabolic processes of most living species. Arginine is an alpha amino acid that is key to the biosynthesis of proteins.
These two amine-based compounds can be detected in the low UV regime. Using a Primesep 100 mixed-mode column and a gradient mobile phase consisting of water and acetonitrile (MeCN) with a gradient of sulfuric acid (H2SO4) as a buffer, L-carnitine and Arginine can be retained, separated, and analyzed. This analysis method can be UV detected at 205 nm.

 

 

Condition

Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 20/80%
Buffer Gradient H2SO4 from 0.1 to 0.5%, 10 min
Flow Rate 1.0 ml/min
Detection UV, 205 nm

 

Description

Class of Compounds
Amino acid
Analyzing Compounds Carnitine,  Arginine

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Arginine
Carnitine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.