Separation type: Liquid Chromatography HILIC
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethylene glycol, PEG 200, PEG 400.
Polyethylene glycol (PEG) has a low toxicity and is used in a variety of products (creams, laxatives, lubricants). When attached to various protein medications, polyethylene glycol allows a slowed clearance of the carried protein from the blood. This makes for a longer acting medicinal effect, reduces toxicity, and allows longer dosing intervals. HILIC separation can be used to determine polyethylene glycol content in various formulations using ELSD, RI detection, or LC/MS detection. HPLC separation of oligomers can be achieved by changing the amount of acetonitrile.
|Primesep AP, 4.6×150 mm, 5 µm, 100A
|MeCN/H2O – 95/5%
|UV, 200 nm, RI
|Class of Compounds
|Ethylene glycol, PEG 200, PEG 400
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.Select options