| Synonyms |
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Applications:
HPLC Method For Analysis of Ethylene Glycol and Polyethylene Glycol (PEG) on Primesep AP Column
September 14, 2021
Separation type: Liquid Chromatography HILIC
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High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethylene glycol, PEG 200, PEG 400.
Polyethylene glycol (PEG) has a low toxicity and is used in a variety of products (creams, laxatives, lubricants). When attached to various protein medications, polyethylene glycol allows a slowed clearance of the carried protein from the blood. This makes for a longer acting medicinal effect, reduces toxicity, and allows longer dosing intervals. HILIC separation can be used to determine polyethylene glycol content in various formulations using ELSD, RI detection, or LC/MS detection. HPLC separation of oligomers can be achieved by changing the amount of acetonitrile.
| Column | Primesep AP, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 95/5% |
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm, RI |
| Class of Compounds |
Diol |
| Analyzing Compounds | Ethylene glycol, PEG 200, PEG 400 |
Application Column
Primesep AP
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsPolyethylene Glycol
HILIC HPLC Separation of Polyethylene Glycol (PEG)
August 22, 2008
Polyethylene glycol (PEG) has low toxicity and is used in a variety of products (creams, laxatives, lubricants). When attached to various protein medications, polyethylene glycol allows a slowed clearance of the carried protein from the blood. This makes for a longer acting medicinal effect, reduces toxicity, and allows longer dosing intervals. HILIC separation of polyethylene glycol can be used to determine polyethylene glycol content in various formulations using ELSD, RI detection, or LC/MS detection. HPLC separation of oligomers can be achieved by changing amount of acetonitrile or buffer pH.
Application Column
Obelisc N
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
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