HPLC Method For Analysis Of Ethoxyquin on Primesep 200 Column

HPLC Method for Ethoxyquin on Primesep 200 by SIELC Technologies

 

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethoxyquin

Ethoxyquin is an antioxidant. It was developed in the 1950s as a preservative to control the breakdown of fats in dry pet food or the formation of peroxide in canned pet foods. Ethoxyquin is also used as an insecticide, herbicide, fungicide, post-harvest dip to prevent scald on apples and pears, plant growth regulator, and anti-degradation agent for rubber.

Ethoxyquin can be retained on the Primesep 200 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and Ammonium Formate (AmFm) buffer. Detection can be achieved with mass spectrometry (MS), evaporative light scattering detection (ELSD), charged aerosol detection (CAD) and UV detected at 260 nm.

Condition
Column Primesep 200, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 50/50%
Buffer AmFm pH 3.0  – 50 mM
Flow Rate 1.0 ml/min
Detection UV, 260 nm

 

Description
Class of Compounds
 Antioxidant, insecticide, herbicide, fungicide
Analyzing Compounds Ethoxyquin

 

 

Application Column

Primesep 200

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Ethoxyquin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.