Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethoxyquin
Ethoxyquin is an antioxidant. It was developed in the 1950s as a preservative to control the breakdown of fats in dry pet food or the formation of peroxide in canned pet foods. Ethoxyquin is also used as an insecticide, herbicide, fungicide, post-harvest dip to prevent scald on apples and pears, plant growth regulator, and anti-degradation agent for rubber. Ethoxyquin can be retained on the Primesep 200 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and Ammonium Formate (AmFm) buffer. Detection can be achieved with mass spectrometry (MS), evaporative light scattering detection (ELSD), charged aerosol detection (CAD) and UV detected at 260 nm.
| Column | Primesep 200, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | AmFm pH 3.0 – 50 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 260 nm |
| Class of Compounds |
Antioxidant, insecticide, herbicide, fungicide |
| Analyzing Compounds | Ethoxyquin |
Application Column
Primesep 200
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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