HPLC Method for Adipic Acid on Newcrom B by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Adipic Acid
Adipic acid (or Hexanedioic acid) is a heavily used, industrial dicarboxylic acid used as a pre-cursor for the mass-production of nylon. It is also used as an ingredient in timed-release drug capsules and as a tart flavoring agent in foods. Adipic acid can be retained and analyzed on a mixed-mode Newcrom B column with a mobile phase consisting of water, Acetonitrile (MeCN), and Sulfuric acid (H2SO4). This analytical method can be UV detected at 200 nm with high resolution and peak symmetry.
| Column | Newcrom B, 4.6 x 150 mm, 3 µm, 100 A |
| Mobile Phase | MeCN/H2O -5/95% |
| Buffer | H2SO4 – 0.02% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm |
| Class of Compounds | Acid, Hydrophilic |
| Analyzing Compounds | Adipic Acid |
Application Column
Newcrom B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Application Analytes:
Adipic AcidApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
