HPLC Method for Uridine on Chromni™ by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Uridine
Uridine is a glycosylated pyrimidine analog with the molecular formula C9H12O6.
Properties:
Appearance: Typically a white or off-white powder.
Molecular weight: ~244.2 g/mol
Solubility: Soluble in water.
Uses: Neuropsychiatric drug.
Uridine can be retained and analyzed using the Chromni™ stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN). Detection is performed using UV.
Column | Chromni™, 4.6 x 150 mm, 3 µm, 100 A, dual ended |
Mobile Phase | MeCN – 95% |
Buffer | None |
Flow Rate | 1.0 ml/min |
Detection | UV 275 nm |
Class of Compounds | Pyrimidine Nucleoside |
Analyzing Compounds | Uridine |
Application Column
Chromni™
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended