HPLC Isocratic Method for Analysis of Sodium Metabisulfite on Primesep SB Column

HPLC Method for Analysis of Sodium metabisulfite on Primesep SB by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of  Sodium metabisulfite

*The metabisulfite ion (S2O52−) is hydrolyzed to bisulfite (HSO3) in water. Sodium metabisulfite is a white crystalline or powder solid. It has many uses, but some of it’s more prominent are: as the source of SO2 in wine, as a bleaching agent in the production of Coconut cream, and added to anesthetic solutions to prevent oxidation to improve the shelf life of the solution. Ascorbic is found naturally in citrus fruits and many vegetables. As a medication, it is used to prevent or treat low levels of vitamin C, since it is that vitamin. Vitamin C is needed to maintain the health of skin, cartilage, teeth, bone, and blood vessels. Ascorbic Acid and Sodium Metabisulfite can be separated, retained, and analyzed on a Primesep SB mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a phosphoric acid (H3PO4) buffer. This analysis method can be detected in the UV 270 nm.

ColumnPrimesep SB 4.6×150 mm 5µm
Mobile PhaseMeCN/H2O – 50/50%
BufferH3PO4 – 0.5%
Flow Rate1.0 ml/min
DetectionUV, 200 nm
Class of Compounds
Acid, Hydrophilic, Ionizable, Disinfectant, Antioxidant, Preservative agent.
Analyzing CompoundsSodium metabisulfite

Application Column

Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Sodium metabisulfite

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.