HPLC Method for Separation Biguanides such as Moroxydine, Metformin, Proguanil and Phenformin on Primesep 100 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Moroxydine, Metformin, Proguanil and Phenformin on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC method for separating Biguanides such as Moroxydine , Metformin, Proguanil and Phenformin on Primesep 100 Column
HPLC Method for Analysis of Metformin, Proguanil hydrochloride, Phenformin, Moroxydine on Primesep 100 Column by SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of Moroxydine , Metformin, Proguanil and Phenformin

Biguanides, including Moroxydine, Metformin, Proguanil, and Phenformin, are a group of organic compounds that share a common structural feature, namely the presence of two guanidine groups.


  • Moroxydine is an antiviral medication used in the treatment of influenza. It is believed to inhibit the early stages of viral replication.


  • Metformin is an oral antidiabetic medication used to treat type 2 diabetes. It works by reducing glucose production in the liver and improving insulin sensitivity in peripheral tissues. Metformin is one of the most widely prescribed drugs for diabetes management.


  • Proguanil is an antimalarial medication often used in combination with other drugs, such as atovaquone. It is used for both prevention and treatment of malaria. Proguanil is a prodrug, and its active form inhibits the development of the malaria parasite in the liver.


  • Phenformin is an antidiabetic biguanide that was previously used to treat type 2 diabetes. However, due to the risk of lactic acidosis (a serious side effect), it has been largely withdrawn from the market in many countries.

These biguanides exhibit various pharmacological activities, and while some are primarily used for antidiabetic purposes, others have applications in antiviral or antimalarial treatments. It’s important to note that the safety and efficacy of these drugs depend on proper medical supervision and adherence to prescribed dosages.

Biguanides such as Moroxydine , Metformin, Proguanil and Phenformin be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and ammonium formate as a buffer. This method allows for detection using UV at 240 nm

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O 70/30%
BufferAmFm pH 3.0  – 30 mM
Flow Rate1.0 ml/min
DetectionUV 240 nm
Samples1. 0.1 mg/ml
2. 0.1 mg/ml
3. 0.1 mg/ml
4. 0.1 mg/ml
Injection volume10 µl
LOD*1. 7 ppb
2. 7 ppb
3. 17 ppb
4. 18 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Analyzing CompoundsMetformin, Proguanil hydrochloride, Phenformin, Moroxydine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Proguanil hydrochloride

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.