Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Succinylcholine
Succinylcholine chloride is a short-acting depolarizing neuromuscular blocker used as a short-term muscle relaxant. Succinylcholine is often used as an adjunct therapy in patients undergoing electroconvulsive shock therapy to control muscle contractions that are induced as a result of the electrical impulses delivered during the procedure. By using a Newcrom AH mixed-mode column with a cation-exchange mechanism, Succinylcholine can be determinate in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN) and a perchloric acid (HClO4) buffer. UV detection at 200 nm.
| Column | Newcrom AH, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 15/85% |
| Buffer | HClO4 – 0.85% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds |
Drug |
| Analyzing Compounds | Succinylcholine |
Application Column
Newcrom AH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsSuccinylcholine chloride
