HPLC Determination of Mycophenolic Acidon Primesep SB Column

HPLC Determination of Mycophenolic Acid
Mycophenolic acid alongside other medicines is used as an immunosuppressant to prevent organ rejection after a transplant. THe compound can be retained in HPLC using Primesep SB mixed-mode column that has both hydrophobic and anion-exchange properties. The analytical method is isocratic and uses the mobile phase of acetonitrile (ACN) in water with phosphoric acid (H3PO4) buffer with UV detection at 226nm.

 

Condition

Column Primesep SB, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 40/60%
Buffer H3PO4- 0.4%
Flow Rate 1.0 ml/min
Detection UV, 226 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophobic, Ionizable, Immunosuppressant
Analyzing Compounds Mycophenolic Acid

 

Application Column

Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Mycophenolic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.