HPLC Method for Analysis of Ketamine Form on Primesep B Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Ketamine on Primesep B by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of Ketamine on Primesep 100 Column by SIELC Technologies

Ketamine is a medication primarily used for induction and maintenance of anesthesia. It also has analgesic and sedative properties.

Ketamine is classified as an arylcyclohexylamine. Chemically, it is known as (RS)-2-(2-chlorophenyl)-2-(methylamino)cyclohexanone.

Ketamine is used primarily as an anesthetic and analgesic in both human and veterinary medicine. It has a unique mechanism of action, acting primarily as an antagonist of the N-methyl-D-aspartate (NMDA) receptor, which is involved in pain transmission and synaptic plasticity.

Beyond its primary medical use, ketamine has also been investigated for its rapid-acting antidepressant effects and is used in certain treatment-resistant cases of depression.

Due to its potential for misuse and the hallucinogenic effects it can produce at sub-anesthetic doses, ketamine is also classified as a controlled substance in many countries.

Ketamine can be retained, and analyzed on a Primesep B mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid as a buffer. This analysis method can be detected using UV at 200 nm.

High Performance Liquid Chromatography (HPLC) Method for Analyses of Ketamine


ColumnPrimesep B, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 5/95%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 200 nm
Peak Retention Time5.56 min
LOD *200 ppb

*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.


Class of CompoundsDrug, arylcyclohexylamine
Analyzing CompoundsKetamine

Application Column

Primesep B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.