HPLC Method for Separation of Glucose and Lysine on Primesep S by SIELC Technologies
Lysine is an essential amino acid typically found in meat, and also cereal grains. It can be manufactured on a large scale by fermenting glucose, one of the most abundant sugars in the world. These two biological compounds can be retained, separated, and analyzed on a normal-phase Primesep S column with a simple isocratic mobile phase consisting of Acetonitrile (MeCN), water (H2O), and Sulfuric acid (H2SO4). This analysis method can be detected via Refraction Index (RI) Detection.
High Performance Liquid Chromatography (HPLC) Method for Separation of Glucose and Lysine
Condition
| Column | Primesep S , 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 75/25% |
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | Refraction Index |
| Peak Retention Time | 4.81 min |
Description
| Class of Compounds | Drug |
| Analyzing Compounds | Glucose, Lysine |
Application Column
Primesep S2
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Application Analytes:
GlucoseLysine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
