HPLC Determination of Fosfomycin on Primesep SB Column

HPLC Determination of Fosfomycin

Fosfomycin is a broad-spectrum antibiotic consisting of an epoxide ring and a phosphite group. It can be retained on a Primesep SB column in about 8 minutes using an isocratic MS-compatible mobile phase method. Primesep SB is a reverse-phase (RP) mixed-mode column with embedded strong basic ion-pairing groups.

 

Condition
Column Primesep SB, 3.2×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 20/80%
Buffer AmFm pH 3.0- 20 mM
Flow Rate 0.5 ml/min
Detection CAD (Corona) MS- compatible mobile phase

 

Description
Class of Compounds
 Drug, Acid, Hydrophilic, Ionizable, Antibiotic
Analyzing Compounds Fosfomycin

 

Application Column

Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Fosfomycin
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.