![FOSFOMYCIN phosphomycin Phosphonomycin 23155-02-4 Veramina Fosfonomycin Fosfocina [(2R,3S)-3-methyloxiran-2-yl]phosphonic acid Fosfomycin sodium Phosphonemycin Phosphomycin disodium salt CHEMBL1757 Fosfomicina Fosfomycine Fosfomycinum](https://www.sielc.com/wp-content/uploads/2019/06/FOSFOMYCIN.png)
| CAS Number | 23155-02-4 |
|---|---|
| Molecular Formula | C3H7O4P |
| Molecular Weight | 138.060 |
| InChI Key | YMDXZJFXQJVXBF-STHAYSLISA-N |
| LogP | -1.91 |
| Synonyms |
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Applications:
HPLC Determination of Fosfomycin on Primesep SB Column
June 7, 2019
Fosfomycin is a broad-spectrum antibiotic consisting of an epoxide ring and a phosphite group. It can be retained on a Primesep SB column in about 8 minutes using an isocratic MS-compatible mobile phase method. Primesep SB is a reverse-phase (RP) mixed-mode column with embedded strong basic ion-pairing groups.
| Column | Primesep SB, 3.2×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | AmFm pH 3.0- 20 mM |
| Flow Rate | 0.5 ml/min |
| Detection | CAD (Corona) MS- compatible mobile phase |
| Class of Compounds |
Drug, Acid, Hydrophilic, Ionizable, Antibiotic |
| Analyzing Compounds | Fosfomycin |
Application Column
Primesep SB
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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Separation of Fosfomycin on Newcrom R1 HPLC column
February 16, 2018
Fosfomycin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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