HPLC Method for Analysis of Chlorogenic acid on Newcrom BH Column by SIELC Technologies
Chlorogenic acid is a natural compound with the chemical formula C16H18O9. It belongs to the polyphenol family, which is abundant in various plants. It is particularly prevalent in coffee beans, fruits, vegetables, and whole grains. One of its primary dietary sources is coffee, where it is found in significant amounts. It is well known for it’s antioxidant properties, which means it can help neutralize free radicals in the body. Free radicals are molecules that can damage cells and contribute to aging and various diseases. Evidence suggests that chlorogenic acid may have a positive impact on blood sugar levels. It may help regulate glucose metabolism, making it of interest in managing diabetes and insulin resistance. Research suggests that chlorogenic acid may have several potential health benefits, including improving heart health by reducing blood pressure and improving blood vessel function. It may also have anti-inflammatory effects. You can find detailed UV spectra of Chlorogenic acid and information about its various lambda maxima by visiting the following link.
Chlorogenic acid can be retained and analyzed on a reversed-phase Newcrom BH column with a mobile phase consisting of water, Acetonitrile (MeCN), and sulfuric acid. This analytical method can be detected with high resolution and peak symmetry at a wavelength of 220 nm using UV detection
Condition
| Column | Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV Vis 325 nm |
| Injection volume: | 2 µL |
| Sample | 1 mg/ml |
| Sample Diluent | MeCN/H2O – 50/50% |
Description
| Class of Compounds | Acid |
| Analyzing Compounds | Chlorogenic acid |
Application Column
Newcrom BH
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
