HPLC Method for Theophylline, Theobromine, Caffeine on Primesep SB by SIELC Technologies

Column | Primesep SB, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
Mobile Phase | EtOH – 10% |
Buffer | H3PO4 – 0.3% |
Flow Rate | 0.5 ml/min |
Detection | UV, 270 nm |
Class of Compounds | Xanthine, Hydrophobic, Ionizable |
Analyzing Compounds | Theophylline, Theobromine, Caffeine |
Application Column
Primesep SB
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
CaffeineTheobromine
Theophylline
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.