CAS Number | 16009-13-5 |
---|---|
Molecular Formula | C34H32ClFeN4O4 |
Molecular Weight | 651.951 |
InChI Key | BTIJJDXEELBZFS-HXFTUNQESA-K |
LogP | 10.4 |
Synonyms |
|
Separation type: Liquid Chromatography Mixed-mode
Hemin, also known under the trade name Panhematin, is a porphyrin containing iron that is used to treat the blood disorder porphyria. This drug can be retained and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Sulfuric acid (H2SO4). This analytical method can be UV detected at 393 nm with high resolution and peak symmetry.
Column | (Column variation not found) |
Mobile Phase | MeCN – 70% |
Buffer | H2SO4 – 0.2% |
Flow Rate | 1.0 ml/min |
Detection | UV 393 nm |
Peak Retention Time | 5.91 |
Class of Compounds | Porphyrin |
Analyzing Compounds | (Compound not found) |
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Hemin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
The Newcrom columns are a family of reverse phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse phase column with low silanol activity.
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