Hemin structural formula

UV- vis absorption spectrum of Hemin

CAS Number16009-13-5
Molecular FormulaC34H32ClFeN4O4
Molecular Weight651.951
  • Hemin
  • Chlorido[3,3'-(7,12-diethenyl-3,8,13,17-tetramethylporphyrin-2,18-diyl-kappa2N21,N23)di(propanoato)(2-)]ironato(2-)
  • Iron, chloro[7,12-diethenyl-3,8,13,17-tetramethyl-21H,23H-porphine-2,18-dipropanoato(2-)-kappaN21,kappaN23]-
  • 16009-13-5
  • Ferrate(2-), chloro[7,12-diethenyl-3,8,13,17-tetramethyl-21H,23H-porphine-2,18-dipropanoato(4-)-?N21,?N22,?N23,?N24]-, dihydrogen, (SP-5-13)-
  • 1,3,5,8-Tetramethyl-2,4-divinylporphine-6,7-dipropionic acid ferrichloride
  • Chloro[dihydrogen 3,7,12,17-tetramethyl-8,13-divinyl-2,18-porphinedipropionato(2-)]iron
  • Chlorohemin
  • Chloroprotoferriheme
  • Chloroprotohemin
  • Ferrate(2-), chloro[7,12-diethenyl-3,8,13,17-tetramethyl-21H,23H-porphine-2,18-dipropanoato(4-)-N21,N22,N23,N24]-, dihydrogen, (SP-5-13)-
  • Ferrate(2-), chloro[7,12-diethenyl-3,8,13,17-tetramethyl-21H,23H-porphine-2,18-dipropanoato(4-)-?N21,?N22,?N23,?N24]-, hydrogen (1:2), (SP-5-13)-
  • Ferric hemin
  • Ferriheme
  • Ferriheme chloride
  • Ferriporphyrin chloride
  • Ferriprotoporphyrin
  • Ferriprotoporphyrin IX
  • Ferriprotoporphyrin IX chloride
  • Hemin chloride
  • Hemin IX
  • Iron(III) protoporphyrin chloride
  • Iron, chloro[dihydrogen 3,7,12,17-tetramethyl-8,13-divinyl-2,18-porphinedipropionato(2-)]-
  • Panhematin
  • Protoferriheme
  • Protohemin
  • Protohemin chloride
  • Protohemin IX
  • Teichmann's crystals
  • EINECS 240-140-1
  • NSC 122707
  • Ferrihaem
  • Hematin
  • Alkaline Hematin D-575
  • Chloroprotoferrihem
  • Teichmanns crystals
  • UNII-743LRP9S7N
  • hemine
  • 1160714-75-9
  • 13408-65-6
  • 15489-47-1
  • 20814-13-5
  • 210561-76-5
  • 35-08-5
  • 58248-97-8
  • 63452-72-2
  • 79266-76-5
  • 83603-95-6


HPLC Method for Analysis of Hemin on Primesep 100 Column


HPLC Method for Analysis of (Compound not found) on (Column not found) by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of Hemin on Primesep 100 Column by SIELC Technologies
HPLC Method for Analysis of (Compound not found) on (Column not found) by SIELC Technologies

Hemin, also known under the trade name Panhematin, is a porphyrin containing iron that is used to treat the blood disorder porphyria. This drug can be retained and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Sulfuric acid (H2SO4). This analytical method can be UV detected at 393 nm with high resolution and peak symmetry.

High Performance Liquid Chromatography (HPLC) Method for Analysis of  (Compound not found)


Column(Column variation not found)
Mobile PhaseMeCN – 70%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 393 nm
Peak Retention Time5.91


Class of CompoundsPorphyrin
Analyzing Compounds(Compound not found)

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Hemin on Newcrom R1 HPLC column

Separation of Hemin on Newcrom R1 HPLC column

Hemin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse phase column with low silanol activity.

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The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.