Flunixin meglumine

Flunixin meglumine structural formula

CAS Number42461-84-7
Molecular FormulaC21H28F3N3O7
Molecular Weight491.464
  • Flunixin meglumine
  • 2-[2-Methyl-3-(trifluoromethyl)anilino]pyridine-3-carboxylic acid--1-deoxy-1-(methylamino)-D-galactitol (1/1)
  • 3-Pyridinecarboxylic acid, 2-[[2-methyl-3-(trifluoromethyl)phenyl]amino]-, compd. with D-galactitol, 1-deoxy-1-(methylamino)- (1:1)
  • 42461-84-7
  • Banamine
  • EINECS 255-836-0
  • Flunixin N-methylglucanine
  • Sch 14714 meglumine
  • Flunixin-S
  • Flumeglumine


HPLC Method for Determination of Flunixin and Meglumine on Primesep 100 Column

September 27, 2022

HPLC Method for Determination of Flunixin and Meglumine on Primesep 100

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Determination of Flunixin Meglumine on Primesep 100 Column by SIELC Technologies
HPLC Method for Determination of Flunixin Meglumine on Primesep 100 by SIELC Technologies

Flunixin is an analgesic and antypyretic nonsteroidal anti-inflammatory drug (NSAID) typically used by veterinarians in bovine and equine treatments. It is typically produced as a Meglumine salt, which helps stabilize and bulk up the medication in its solid form. Flunixin and Meglumine can be retained, separated, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple isocratic mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer.  This analysis method can be UV detected at 252 nm (Flunixin) with high resolution and peak symmetry and is compatible with Mass Spectrometry (MS), ELSD, and CAD (Flunixin and Melumine).


Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN – 60%
Buffer TFA – 0.2%
Flow Rate 1.0 ml/min
Detection ELSD, 50C, UV 252 nm



Class of Compounds Drug
Analyzing Compounds Flunixin Meglumine

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Flunixin meglumine
Meglumine (N-Methyl-D-glucamine)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Flunixin meglumine on Newcrom R1 HPLC column

February 16, 2018
Separation of Flunixin meglumine on Newcrom C18 HPLC column

Flunixin meglumine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Flunixin meglumine
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.