| CAS Number | 4205-90-7 |
|---|---|
| Molecular Formula | C9H9Cl2N3 |
| Molecular Weight | 230.090 |
| InChI Key | GJSURZIOUXUGAL-UHFFFAOYSA-N |
| LogP | 1.59 |
| Synonyms |
|
Applications:
HPLC Method for Analysis of Clonidine on Primesep B Column
March 29, 2024
HPLC Method for Analysis of Clonidine on Primesep B by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Clonidine
Clonidine is a medication primarily used to treat high blood pressure (hypertension).
Clonidine is primarily prescribed for the treatment of hypertension, especially in cases where other antihypertensive medications have not been effective or well-tolerated.
Clonidine is available in various formulations, including tablets, patches, and oral suspension.
Clonidine can be retained, and analyzed using a Primesep B mixed-mode stationary phase column. The analysis utilizes an gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as a buffer. Detection is achieved using UV 290 nm
| Column | Primesep B, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | Gradient MeCN/H2O – 40-80%, 10 min |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 290 nm |
| Class of Compounds | Sulfonamides, sulphonamides, loop diuretics |
| Analyzing Compounds | Clonidine |
Application Column
Primesep B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Separation of Clonidine hydrochloride on Newcrom R1 HPLC column
May 16, 2018
Clonidine hydrochloride can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsClonidine
Separation of Clonidine on Newcrom R1 HPLC column
February 16, 2018
Clonidine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options