BIST™ Application Examples
Here we demonstrate three examples of separation of basic polar and non-polar molecules, that in a typical reverse phase chromatography settings, are either difficult to retain or produce poor peak shape and efficiency. BIST™ conditions can, instead, produce high efficiency, retention and symmetrical peak shape.
Multi-Charged Molecule Analysis
BIST™ can also separate structural isomers of multiple-charged compounds, such as sulfated cyclodextrin,
with a relatively simple gradient method on a short column. Previous methods for analysis of similar compounds
can require much longer columns, higher concentration buffers, and do not provide complete separation
of the constituent isomers.
The power of BIST™ lies in its ability to do these kinds of separations on short columns with simple mobile phases and low concentration buffers. Polymers with charged units, like polylysine, are difficult to separate using ion-exchange (IE) chromatography due to very strong and often irreversible interactions with the oppositely-charged stationary phase of the column. Usually, an extremely high concentration of the buffer, needs to be used to generate an ion-exchange process. This high buffer concentration isn’t usually desirable because of the higher viscosity of the MP and the likely formation of salt in the components of the HPLC system. With BIST™, polymers, like these, can be retained and analyzed with a small concentration of the buffer in the mM range using simple gradient of water.
Colistin is another multi-charged molecule with structural isomers that BIST™ can retain. All that’s needed is the typical high-MeCN
concentration MP and a doubly-charged H2SO4 buffer. With this MP, the two isomers can be separated different only in one methyl group.
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The information and disclosures contained herein were originated by and are the property of SIELC Technologies Corporation and may not be copied, distributed, republished, translated, uploaded, posted, transmitted, stored, or otherwise used in any shape, manner, or form by any means without the specific prior written permission of SIELC Technologies Corporation. Modification or use of this material for any reason shall be construed as a violation of the property rights of SIELC Technologies Corporation. SIELC Technologies Corporation reserves all rights, including without limitation all patent, proprietary design, manufacturing, reproduction, use, advertising, and sales rights thereto, and to any articles disclosed herein, except to the extent such rights are granted to others by law or by SIELC Technologies Corporation. BISTTM is a registered trademark of SIELC Technologies Corporation. U.S. Patents Pending. All data were obtained in SIELC Technologies labs.
