Alltesta HPLC Method for Furosemide on Primesep B4 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Furosemide
Furosemide is a loop diuretic commonly used to treat conditions like edema (fluid retention) and hypertension (high blood pressure).
Furosemide can be retained, and analyzed using a Primesep B mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as a buffer. Detection is achieved using UV at 275 nm
| Column | Primesep B4, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H3PO4 -0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 275 nm |
| Class of Compounds | Diuretic |
| Analyzing Compounds | Furosemide |
You can view examples of chromatograms obtained using the Allesta instrument and Sielc columns by clicking here.
Application Column
Primesep B4
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Application Analytes:
FurosemideApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
