HPLC Retention Control of Antibiotics (Metronidazole and Norfloxacine) in Toothpaste

 

Commercially available toothpaste has been known to include antibiotics. To demonstrate the retention control on the mixed-mode column Primesep 100, toothpaste was spiked with the antibiotics metronidazole and norfloxacin. In a 13 minute method, retention of the antibiotics was controlled by adjustment in ratio of organic in the mobile phase. This method demonstrates the flexibility of using mixed-mode columns such as Primesep 100.

 

Condition

Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

Description

Class of Compounds
Drug, Antibiotics, Hydrophilic, Ionizable
Analyzing Compounds Metronidazole, Norfloxacine

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Metronidazole
Norfloxacin

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.