CAS Number133107-64-9
Molecular FormulaC257H389N65O77S6
Molecular Weight5813.680
  • Insulin lispro
  • 133107-64-9
  • Insulin, (human), 28B-L-lysine-29B-L-proline-
  • Insulin (ox), 8a-L-threonine-10a-L-isoleucine-28b-L-lysine-29b-L-proline-30b-L-threonine
  • (Lys(B28),pro(B29))-human insulin
  • Humalog
  • Humalog mix50/50
  • Humalog mix75/25
  • Liprolog


HPLC Separation of Human and Synthetic Insulins on Promix Column


Insulin is a protein that regulates carbohydrates and fat metabolism. Insulin is a peptide hormone consisting of 51 amino acids and has molecular weight of 5808 Da. Bovine insulin differs from human in only three amino acid residues, and porcine insulin in one. Difference in structures is very minimal and the insulin cannot be resolve on reversed-phase column. Mixed-mode approach allows to use small difference in hydrophobic and ionic properties and separate insulins with close structures. Normal human insulin, synthetic insulin Humalog, and synthetic insulin Lantus are separated on a Promix MP mixed-mode HPLC column with LC/MS-compatible conditions. Method does not require use of ion-pairing reagent. Method is also compatible with preparative chromatography and can be used for isolation of insulin on a large scale.

Application Column

Promix MP

The Promix family of mixed-mode columns offer an alternative chromatography technology to deliver efficient resolution of peptides and proteins. The technology is based on a combination of both hydrophobic and ionic interactions. This approach is possible due to a new type of separation media: a chemical combination of hydrophobic and ionic functional groups on a ligand bonded to a silica support. With this phase, unparalleled selectivity and peak capacity can be achieved. Independent adjustment of the amount of buffer and organic modifier creates an infinite number of separation conditions that are suitable for many types of bio-molecules.

Select options
Application Analytes:

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to so we can ensure you get optimal results for your compound/s of interest.