Insulin is a protein that regulates carbohydrates and fat metabolism. Insulin is a peptide hormone consisting of 51 amino acids and has molecular weight of 5808 Da. Bovine insulin differs from human in only three amino acid residues, and porcine insulin in one. Difference in structures is very minimal and the insulin cannot be resolve on reversed-phase column. Mixed-mode approach allows to use small difference in hydrophobic and ionic properties and separate insulins with close structures. Normal human insulin, synthetic insulin Humalog, and synthetic insulin Lantus are separated on a Promix MP mixed-mode HPLC column with LC/MS-compatible conditions. Method does not require use of ion-pairing reagent. Method is also compatible with preparative chromatography and can be used for isolation of insulin on a large scale.
The Promix family of mixed-mode columns offer an alternative chromatography technology to deliver efficient resolution of peptides and proteins. The technology is based on a combination of both hydrophobic and ionic interactions. This approach is possible due to a new type of separation media: a chemical combination of hydrophobic and ionic functional groups on a ligand bonded to a silica support. With this phase, unparalleled selectivity and peak capacity can be achieved. Independent adjustment of the amount of buffer and organic modifier creates an infinite number of separation conditions that are suitable for many types of bio-molecules.Select options
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