HPLC Separation of Maleic Acid and Pentaerythritol

  Application Notes: Maleic acid is a small hydrophilic acid, which is poorly retained in reversed-phase chromatography. Pentaerythritol is hydrophilic neutral molecule. A miixed-mode HILIC/anion-exchange method was developed on Obelisc N HPLC column. The retention time for maleic acid can be controlled by amount of ACN, buffer pH, and buffer concentration. Pentaerythritol retention time is only controlled by the amount of acetonitrile. The method is LC/MS and ELSD compatible. Application Columns: Obelisc N Application compounds: Maleic Acid, Pentaerythritol Detection technique: LC/MS, ELSD/CAD 


Column Obelisc N, 3.2x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 95/5%
Buffer AmFm pH 3.0- 5 mM
Flow Rate 0.5 ml/min
Detection ELSD


Class of Compounds Acid, Hydrophilic, Ionizable
Analyzing Compounds Maleic Acid, Pentaerythritol
Application Analytes:
Maleic Acid