HPLC Separation of Heparines and Pentosan

Heparin and pentosan are highly-sulfated glucosaminoglucan, widely used in pharmaceutical industry. Both molecules carry a high negative density attributed to multiple sulfate groups. Due to a very polar and polymeric nature of these compounds, separating and quantifying them is a very difficult task. Heparin and its salt, along with pentosan, are separated on a Primesep N column in HILIC/anion-exclusion mode. Method can be used for quantitation of heparin and pentosan in biofluids and formulations. Elution is monitored by ELSD, CAD or LC/MS.

Application Column

Primesep N

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.