HPLC Separation of Glyphosate Production Intermediates

Glyphosate is a broad spectrum herbicide used to kill weeds. It is the most used herbicide. Glyphosate is an aminophosphonic analogue of the natural amino acid glycine. Glyphosate and its intermediates are very polar ionic compounds derived from glycine. Neither of intermediates can be retained on traditional reversed-phase columns. Two methods for glyphosate intermediates were developed on Primesep B, Primesep B2 and Primesep 100 columns.


Column Primesep B, Primesep B2, Primesep 100,  4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA, H2SO4
Flow Rate 1.0 ml/min
Detection ELSD


Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Glyphosate, PMIDAA, IDA
Application Analytes:
DEA (Diethanolamine)
IDA (Iminodiacetic acid)
Iminodiacetic Acid
PMIDA (Phosphonomethyliminodiacetic acid)
Phosphonomethyliminodiacetic Acid