Mixture of polar acidic and basic compounds is separated on a Primesep AB mixed-mode HPLC column. Dopamine and tyrosine are retained by combination of reversed-phase and cation-exchange mechanisms. Maleic acid is retained by anion-exchange mechanism, and benzoic acid is retained by reversed-phase mechanism. Primesep AB is a trimodal column with a C12 hydrophobic chain and cation-exchange and anion exchange groups on the surface. Method utilizes UV detection but can be used with other detection techniques (ELSD, LC/MS, Corona).
| Column | Primesep AB, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | TFA – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 210 nm |
| Class of Compounds |
Drug, Acid, Monocarboxylic acid, Hydrophilic, Ionizable, Hormone |
| Analyzing Compounds | Tyrosine, Dopamine, Maleic Acid, Benzoic Acid |
Application Column
Primesep AB
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsDopamine
Maleic Acid
Tyrosine
