| CAS Number | 573-20-6 |
|---|---|
| Molecular Formula | C15H14O4 |
| Molecular Weight | 258.27 |
| InChI Key | RYWSYCQQUDFMAU-UHFFFAOYSA-N |
| LogP | 3 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Acetomenaphthone
February 18, 2026
Access the UV-Vis Spectrum SIELC Library
If you are looking for optimized HPLC method to analyze Vitamin K4 (Acetomenaphthone) check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Analysis of Vitamin K4 (Acetomenaphthone) and Vitamin K1 (Phytomenadione) on Lipak Column
December 18, 2024
HPLC Method for Vitamin K1 (Phytomenadione), Vitamin K4 (Acetomenaphthone) on Lipak by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Vitamin K1 (Phytomenadione), Vitamin K4 (Acetomenaphthone)
Vitamin K1 (phylloquinone) and Vitamin K4 (menadiol or menadiol diacetate) are essential compounds involved in blood clotting and bone health. Vitamin K1, found in green leafy vegetables, is the natural form crucial for synthesizing clotting factors in the liver. Vitamin K4, a synthetic derivative, is used in supplements and treatments due to its stability and bioavailability. Both forms contribute to activating proteins necessary for calcium regulation, preventing vascular calcification, and supporting overall cardiovascular and skeletal health.
Vitamin K1 (Phytomenadione), Vitamin K4 (Acetomenaphthone) can be retained, and analyzed using a Lipak mixed-mode stationary phase column. The analysis utilizes an gradient method with a mobile phase consisting of water, methanol (MeOH), ethanol (EtOH), ammonium formate and formic acid as a buffer. Detection is achieved using UV 270 nm.
| Column | Lipak, 3.2 x 100 mm, 3 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeOH/H2O/EtOH – 40/60/0-0/0/100% in 5 min, 5 min hold |
| Buffer | AmFm– 10 mM, FA – 0.05% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 270 nm |
| Class of Compounds | Fat Soluble Vitamins |
| Analyzing Compounds | Vitamin K1 (Phytomenadione), Vitamin K4 (Acetomenaphthone) |
Application Column
Lipak
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended
Vitamin K4 (Acetomenaphthone)
Separation of Acetomenaphthone on Newcrom R1 HPLC column
February 16, 2018
Acetomenaphthone can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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