Hydroxyproline

Hydroxyproline

Molecular FormulaC5H9NO3
Molecular Weight131.13
InChI KeyPMMYEEVYMWASQN-DMTCNVIQSA-N
LogP-3.3
Synonyms
  • L-Hydroxyproline
  • hydroxyproline
  • trans-4-Hydroxyproline
  • (2S,4R)-4-Hydroxypyrrolidine-2-carboxylic acid
  • L-4-hydroxyproline
  • H-Hyp-OH
  • hydroxy-L-proline
  • Hypro
  • 4-hydroxyproline
  • (4R)-4-hydroxy-L-proline
  • 4-Hydroxy-L-proline
  • trans-L-Hydroxyproline
  • 4-Hydroxy-2-pyrrolidinecarboxylic acid
  • trans-Hydroxyproline
  • L-Proline, 4-hydroxy-, (4R)-
  • Hydroxyproline (VAN)
  • Ls-Hydroxyproline
  • UNII-RMB44WO89X
  • Proline, 4-hydroxy-, L-
  • delta-Hydroxyproline
  • 4-L-Hydroxyproline
  • Proline, 4-hydroxy- (VAN)
  • L-Proline, 4-hydroxy-
  • Proline, 4-hydroxy-
  • RMB44WO89X
  • CHEMBL352418
  • CHEBI:18095
  • (2S,4R)-trans-4-hydroxyproline
  • Trans-4-Hydroxy-L-Proline, 99+%
  • (2S,4R)-(-)-4-Hydroxy-2-Pyrrolidinecarboxylic Acid
  • L-Proline, 4-hydroxy-, trans-
  • L-Proline, 4-hydroxy-, trans- (9CI)
  • (2S,4R)-4-hydroxy-2-pyrrolidinecarboxylic acid
  • (2S,4R)-4-hydroxyproline
  • EINECS 200-091-9
  • MFCD00064320
  • NSC 46704
  • SMR000857104
  • trans-4-hydroxy-proline
  • Hyp
  • hydroxiproline
  • oxyproline
  • hydroxy-proline
  • trans-4-hydroxy-D,L-proline
  • Pyrrolidin-4-ol-2-carboxylic acid
  • L-Hydroxyproline;
  • h-l-hyp-oh
  • H-trans-Hyp-OH
  • hydroxyproline, l-
  • Hydroxyproline,(l)
  • L-Proline, trans-
  • (4r)-hydroxyproline
  • 4(R)-hydroxyproline
  • (2s,4r)-(-)-4-hydroxypyrrolidine-2-carboxylic acid
  • .delta.-Hydroxyproline
  • L-Hydroxyproline,(S)
  • L-4-transhydroxyproline
  • L-threo-4-hydroxyproline
  • trans-4-hydroxyL-proline
  • Trans4-hydroxy-L-proline
  • bmse000123
  • bmse000966
  • L-trans-4-hydroxy-proline
  • trans-4-hydroxy(L)proline
  • trans-4-hydroxyl-l-proline
  • SCHEMBL21185
  • KSC269I9J
  • MLS001332463
  • MLS001332464
  • trans-4-hydroxy-(L)-proline
  • (2S, 4R)-4-hydroxyproline
  • GTPL4704
  • (2s,4r)-4-hydroxy-l-proline
  • (2S, 4R)-4-hydroxy-proline
  • CTK1G9494
  • DTXSID10883225
  • PMMYEEVYMWASQN-DMTCNVIQSA-N
  • ZINC901791
  • ACT08225
  • KS-00000O2S
  • NSC46704
  • Z-TRANS-4-HYDROXY-L-PROLINE
  • ANW-31245
  • BDBM50357233
  • CH-388
  • NSC-46704
  • SBB004222
  • trans-4-Hydroxy-L-proline, >=99%
  • AKOS007930607
  • AB02585
  • AC-2249
  • AM81829
  • CS-W008928
  • DB08847
  • LS30143
  • MCULE-5389747201
  • PS-5807
  • KS-0000050M
  • (2S,4R)-2-carboxy-4-hydroxypyrrolidine
  • 54733-36-7
  • BP-10638
  • BR-26056
  • HY-40135
  • NCI60_004102
  • AB0063025
  • AB1001753
  • TX-018154
  • FT-0634378
  • FT-0639142
  • C01157
  • H-7290
  • M-5819
  • M03214
  • trans-4-Hydroxy-L-proline, analytical standard
  • 33779-EP2272822A1
  • 33779-EP2272972A1
  • 33779-EP2272973A1
  • 33779-EP2277867A2
  • 33779-EP2277872A1
  • 33779-EP2277877A1
  • 33779-EP2277898A2
  • 33779-EP2280003A2
  • 33779-EP2295055A2
  • 33779-EP2311453A1
  • (2S, 4R)-4-hydroxy-2-pyrrolidinecarboxylic acid
  • (2S,4R)-4-hydroxy-pyrrolidine-2-carboxylic acid
  • 064H320
  • 4-(R)-hydroxy-pyrrolidine-2-(S)-carboxylic acid
  • (2S, 4R)-4-hydroxy-2-pyrrolidine-carboxylic acid
  • J-525022
  • Q27089020
  • trans-4-Hydroxy-L-proline, BioXtra, >=99.0% (NT)
  • F8889-8652
  • Z1259273356
  • F05487BD-FA1D-4C80-83AD-3A91E2078031
  • trans-4-Hydroxy-L-proline, microbial, cell culture tested
  • trans-4-Hydroxy-L-proline, Vetec(TM) reagent grade, 99%
  • UNII-9J17BCS69U component PMMYEEVYMWASQN-DMTCNVIQSA-N
  • UNII-SZB83O1W42 component PMMYEEVYMWASQN-DMTCNVIQSA-N
  • trans-4-Hydroxy-L-proline, BioReagent, suitable for cell culture, >=98.5%

Applications:

New HPLC Amino Acids Separation Compatible With Carbon Dating Technique

February 11, 2020

 

 

Hydroxyproline seems to be the most promising amino acid used in carbon dating when isolated from bone collagen. Separation of amino acids is challenging, especially without the use of ions or inorganic buffers that can interfere with Mass spectrometer (MS) or contaminate the sample with modern carbon. Amino acids are also not retained in reverse-phase chromatography. The ideal solution would be using water only to separate the amino acids. This would allow a direct coupling to MS. We were able to separate hydroxyproline from proline and other simple amino acids like glycine and alanine in HPLC on Newcrom AH column using water only as a mobile phase. Using water also allowed UV detection at 205 nm which can’t be done if using a buffer based on acetic or formic acid.
See more information on radiocarbon dating here.
The same method can be modified to get symmetrical peaks and higher efficiency if a mobile phase with ionic modifier such as formic acid is used.

Condition
Column Newcrom AH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Flow Rate 1.0 ml/min
Detection UV, 205 nm, CAD

 

Description
Class of Compounds
 Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Alanine, Glycine, Proline, Hydroxyproline

 

Application Column

Newcrom AH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Alanine
D-Alanine
Glycine
Hydroxyproline
Proline
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.