| CAS Number | 59-67-6 |
|---|---|
| Molecular Formula | C6H5NO2 |
| Molecular Weight | 123.112 |
| InChI Key | PVNIIMVLHYAWGP-UHFFFAOYSA-N |
| LogP | 0.36 |
| Synonyms |
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Applications:
USP Methods for the Analysis of Niacin with the Legacy L1 Column
June 21, 2012
Niacin (vitamin B3, nicotinic acid) is one of the essential vitamins. The water-soluble vitamin was analyzed by reversed-phase chromatography on the C18 reversed-phase HPLC column, Legacy L1. This column is classified according to USP as a L1-type of column. It can be used for the analysis of various drugs, vitamins, and chemicals according to United States Pharmacopeia.
| Column | Legacy L1, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | Hexanesulfonate/MeOH/MeCN/AcOH –78/14/7/2 |
| Buffer | Hexanesulfonate |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds |
Drug, Cholesterol medication, Vitamin B3, Hydrophobic, Ionizable |
| Analyzing Compounds | Vitamin B3, Niacin |
Application Column
Legacy L1
SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.
Select options
HPLC Method for Separation of Vitamins Group B such as Nicotinic Acid, Pyridoxine, Niacinamide, Pantothenic Acid, Riboflavin on Obelisc N Column
August 22, 2008
Separation of vitamins group B is achieved on Obelisc N column in HILIC mixed-mode. Vitamins of this group are different in polarity and ionic properties. Retention and separation is achieved by optimization of amount of ACN, buffer and buffer pH. Combination of UV and ELSD detection is used to monitor HPLC separation. B vitamins are water-soluble vitamins that play an important role in cell metabolism. Supplements containing all six are generally referred to as a vitamin B complex. Individual B vitamin supplements are referred to by the specific name of each vitamin. This method can be used to analyze individual B vitamins as well as vitamin B complex. Isolation of impurities as well as degradation products is possible by preparative chromatography.
| Column | Obelisc N , 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmAC pH 4.5 – 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm, ELSD |
| Class of Compounds |
Drug, Vitamin B₆, Hydrophobic, Ionizable |
| Analyzing Compounds | Pyridoxine, Niacinamide, Pantothenic acid, Riboflavin |
Application Column
Obelisc N
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsVitamin B1 (Thiamine)
Vitamin B2 (Riboflavin)
Vitamin B3 (Niacin)
Vitamin B5 (Pantothenic Acid)
Vitamin B6 (Pyridoxine)
UV Detection
