USP Methods for the Analysis of Niacin with the Legacy L1 Column

Niacin (vitamin B3, nicotinic acid) is one of the essential vitamins. The water-soluble vitamin was analyzed by reversed-phase chromatography on the C18 reversed-phase HPLC column, Legacy L1. This column is classified according to USP as a L1-type of column. It can be used for the analysis of various drugs, vitamins, and chemicals according to United States Pharmacopeia.


Column Legacy L1, 4.6×150 mm, 5 µm, 100A
Mobile Phase Hexanesulfonate/MeOH/MeCN/AcOH –78/14/7/2
Buffer Hexanesulfonate
Flow Rate 1.0 ml/min
Detection UV, 250 nm



Class of Compounds
Drug, Cholesterol medication, Vitamin B3, Hydrophobic, Ionizable
Analyzing Compounds Vitamin B3, Niacin


Application Column

SIELC Legacy L1 HPLC column

Legacy L1

SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.

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Application Analytes:
Vitamin B3 (Niacin)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to so we can ensure you get optimal results for your compound/s of interest.