| CAS Number | 59-43-8 |
|---|---|
| Molecular Formula | C12H17ClN4OS |
| Molecular Weight | 300.810 |
| InChI Key | MYVIATVLJGTBFV-UHFFFAOYSA-M |
| LogP | -1.42 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Water-Soluble Vitamins (B1, B2, B3, B6) on Primesep 100 Column
August 28, 2024
High Performance Liquid Chromatography (HPLC) Method for Analysis of Water-Soluble Vitamins (B1, B2, B3, B6) on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Vitamin B1 (Thiamine), Vitamin B2 (Riboflavin), Nicotinamide, Vitamin B6 (Pyridoxine), Pyridoxamine, Pyridoxal Phosphate
Water-Soluble Vitamins (B1, B2, B3, B6) can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an gradient method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 300 nm.
You can find detailed UV spectra of Vitamin B1 (Thiamine), Vitamin B2 (Riboflavin), Nicotinamide, Vitamin B6 (Pyridoxine), Pyridoxamine, Pyridoxal Phosphate and information about its various lambda maxima by visiting the following links: Vitamins (B1, B2, B3, B6)
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN – 10% |
| Buffer | Gradient H2SO4 -0.1-0.5% in 10 min |
| Flow Rate | 1.0 ml/min |
| Detection | UV 270 nm |
| Class of Compounds | Water-Soluble Vitamins (B1, B2, B3, B6) |
| Analyzing Compounds | Vitamin B1 (Thiamine), Vitamin B2 (Riboflavin), Nicotinamide, Vitamin B6 (Pyridoxine), Pyridoxamine, Pyridoxal Phosphate |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Pyridoxal Phosphate
Pyridoxamine
Vitamin B1 (Thiamine)
Vitamin B2 (Riboflavin)
Vitamin B6 (Pyridoxine)
HPLC Method for Analysis of Thiamine on Primesep 100 Column
August 13, 2024
High Performance Liquid Chromatography (HPLC) Method for Analysis of Vitamin B1 (Thiamine) on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Vitamin B1 (Thiamine)
Thiamine, also known as vitamin B1, is an essential nutrient that plays a crucial role in energy metabolism and the proper functioning of the nervous system. It helps the body convert carbohydrates into energy and is vital for the growth, development, and function of cells.
Vitamin B1 (Thiamine) can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 250 nm.
You can find detailed UV spectra of Vitamin B1 (Thiamine) and information about its various lambda maxima by visiting the following link.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN – 10% |
| Buffer | H2SO4 -0.4% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 250 nm |
| Samples | 1 mg/ml in H2O |
| Injection volume | 1 µl |
| LOD* | 60 ppb (250 nm) |
| Class of Compounds | Vitamins |
| Analyzing Compounds | Vitamin B1 (Thiamine) |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
UV-Vis Spectrum of Thiamine
July 23, 2024
UV-Vis Spectrum of Thiamine. Absorption Maxima: 246 nm.
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Separation of Vitamins Group B such as Nicotinic Acid, Pyridoxine, Niacinamide, Pantothenic Acid, Riboflavin on Obelisc N Column
August 22, 2008
Separation of vitamins group B is achieved on Obelisc N column in HILIC mixed-mode. Vitamins of this group are different in polarity and ionic properties. Retention and separation is achieved by optimization of amount of ACN, buffer and buffer pH. Combination of UV and ELSD detection is used to monitor HPLC separation. B vitamins are water-soluble vitamins that play an important role in cell metabolism. Supplements containing all six are generally referred to as a vitamin B complex. Individual B vitamin supplements are referred to by the specific name of each vitamin. This method can be used to analyze individual B vitamins as well as vitamin B complex. Isolation of impurities as well as degradation products is possible by preparative chromatography.
| Column | Obelisc N , 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmAC pH 4.5 – 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm, ELSD |
| Class of Compounds |
Drug, Vitamin B₆, Hydrophobic, Ionizable |
| Analyzing Compounds | Pyridoxine, Niacinamide, Pantothenic acid, Riboflavin |
Application Column
Obelisc N
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsVitamin B1 (Thiamine)
Vitamin B2 (Riboflavin)
Vitamin B3 (Niacin)
Vitamin B5 (Pantothenic Acid)
Vitamin B6 (Pyridoxine)
UV Detection
HPLC Separation of Vitamin B
April 3, 2004
Primesep C separates B vitamins with baseline resolution by a combination of cation exchange, complex formation, and hydrophobic interactions. Vitamin B1 (thiamine), vitamin B2 (riboflavin), and vitamin B6 (pyridoxine) are separated with a mobile phase of water, acetonitrile (MeCN, ACN) and triethylamine (TEA) phosphate with UV detection at 280 nm.
| Column | Primesep C , 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O -10/90% |
| Buffer | NEt3H3PO4 Ph 3.0 – 20 Mm |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 280 nm |
| Class of Compounds |
Drug, Vitamin B₆, Hydrophobic, Ionizable |
| Analyzing Compounds | Pyridoxine, Thiamine, Riboflavin |
Application Column
Primesep C
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsVitamin B1 (Thiamine)
Vitamin B2 (Riboflavin)
Vitamin B6 (Pyridoxine)
Vitamins
