| CAS Number | 58-55-9 |
|---|---|
| Molecular Formula | C7H8N4O2 |
| Molecular Weight | 180.168 |
| InChI Key | ZFXYFBGIUFBOJW-UHFFFAOYSA-N |
| LogP | -0.0200 |
| Synonyms |
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Applications:
HPLC Determination of Caffeine, Theophylline and Theobromine in Tea on Primesep SB Column
December 17, 2020
| Column | Primesep SB, 3.2×100 mm, 5 µm, 100A |
| Mobile Phase | EtOH – 10% |
| Buffer | H3PO4 – 0.3% |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Xanthine, Hydrophobic, Ionizable |
| Analyzing Compounds | Caffeine, Theophylline, Theobromine |
Application Column
Primesep SB
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsTheobromine
Theophylline
Separation of Theophylline monohydrate on Newcrom R1 HPLC column
May 16, 2018
Theophylline monohydrate can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsTheophylline monohydrate
Effect of Acid on Separation of Xanthines
July 6, 2003
Primesep B separates xanthines such as theobromine, dimethylxanthine, theophylline by a combination of reversed-phase and ion-exchange mechanisms. The anion-exchange properties of the column allow retention of hydrophilic xanthines, and the reversed-phase properties retain hydrophobic compounds. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and either trifluoroacetic acid (TFA), perchloric acid (HClO4), or sulfuric acid (H2SO4) and UV detection.
| Column | Primesep B |
| Mobile Phase | MeCN/H2O |
| Buffer | TFA , HClO4, H2SO4 |
| Flow Rate | 1.0 ml/min |
| Detection |
| Class of Compounds |
Drug, Acid, Hydrophilic, Ionizable, Hormone |
| Analyzing Compounds | Theobromine, 1,7 – Dimethylxanthine, Theophyline |
Application Column
Primesep B
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsTheobromine
Theophylline
Theophylline monohydrate
