CAS Number | 83-67-0 |
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Molecular Formula | C7H8N4O2 |
Molecular Weight | 180.168 |
InChI Key | YAPQBXQYLJRXSA-UHFFFAOYSA-N |
LogP | -0.780 |
Synonyms |
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Applications:
HPLC Determination of Caffeine, Theophylline and Theobromine in Tea on Primesep SB Column
December 17, 2020
Column | Primesep SB, 3.2×100 mm, 5 µm, 100A |
Mobile Phase | EtOH – 10% |
Buffer | H3PO4 – 0.3% |
Flow Rate | 0.5 ml/min |
Detection | UV, 270 nm |
Class of Compounds |
Xanthine, Hydrophobic, Ionizable |
Analyzing Compounds | Caffeine, Theophylline, Theobromine |
Application Column
Primesep SB
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsTheobromine
Theophylline
Effect of Acid on Separation of Xanthines
July 6, 2003
Primesep B separates xanthines such as theobromine, dimethylxanthine, theophylline by a combination of reversed-phase and ion-exchange mechanisms. The anion-exchange properties of the column allow retention of hydrophilic xanthines, and the reversed-phase properties retain hydrophobic compounds. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and either trifluoroacetic acid (TFA), perchloric acid (HClO4), or sulfuric acid (H2SO4) and UV detection.
Column | Primesep B |
Mobile Phase | MeCN/H2O |
Buffer | TFA , HClO4, H2SO4 |
Flow Rate | 1.0 ml/min |
Detection |
Class of Compounds |
Drug, Acid, Hydrophilic, Ionizable, Hormone |
Analyzing Compounds | Theobromine, 1,7 – Dimethylxanthine, Theophyline |
Application Column
Primesep B
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsTheobromine
Theophylline
Theophylline monohydrate