| CAS Number | 94-07-5 |
|---|---|
| Molecular Formula | C9H13NO2 |
| Molecular Weight | 167.208 |
| InChI Key | YRCWQPVGYLYSOX-UHFFFAOYSA-N |
| LogP | -0.450 |
| Synonyms |
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Applications:
HPLC Separation of Octopamine and Synephrine on Primesep 200 Column
April 26, 2011
Octopamine is an analog of norepinephrine in invertebrates that’s an important neurotransmitter and hormone. Synephrine is another molecule that has a similar structure to norepinephrine and is a very strong agonist of octopamine receptors in invertebrates. Both compounds can be baseline separated with an isocratic method in 30/70 Acetonitrile (ACN) and water mobile phase with a 30mM Ammonium Formate (AmFm) buffer at pH 3. UV detection 270nm.
| Column | Primesep 200, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | AmFm pH 3.0- 30 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Drug, Acid, Hydrophilic, Ionizable,Neurotransmitter |
| Analyzing Compounds | Octopamine, Synephrine |
Application Column
Primesep 200
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsSynephrine
